Complete chloroplast genome sequencing and phylogeny of wild Atractylodes lancea from Yuexi, Anhui province / 中国中药杂志

This study investigated the choroplast genome sequence of wild Atractylodes lancea from Yuexi in Anhui province by high-throughput sequencing, followed by characterization of the genome structure, which laid a foundation for the species identification, analysis of genetic diversity, and resource conservation of A. lancea. To be specific, the total genomic DNA was extracted from the leaves of A. lancea with the improved CTAB method. The chloroplast genome of A. lancea was sequenced by the high-throughput sequencing technology, followed by assembling by metaSPAdes and annotation by CPGAVAS2. Bioiformatics methods were employed for the analysis of simple sequence repeats(SSRs), inverted repeat(IR) border, codon bias, and phylogeny. The results showed that the whole chloroplast genome of A. lancea was 153 178 bp, with an 84 226 bp large single copy(LSC) and a 18 658 bp small single copy(SSC) separated by a pair of IRs(25 147 bp). The genome had the GC content of 37.7% and 124 genes: 87 protein-coding genes, 8 rRNA genes, and 29 tRNA genes. It had 26 287 codons and encoded 20 amino acids. Phylogenetic analysis showed that Atractylodes species clustered into one clade and that A. lancea had close genetic relationship with A. koreana. This study established a method for sequencing the chloroplast genome of A. lancea and enriched the genetic resources of Compositae. The findings are expected to lay a foundation for species identification, analysis of genetic diversity, and resource conservation of A. lancea.

A simple replacement of tungsten filament hot cathodes by DC heated LaB6 rod and its noise characteristics with laser-induced fluorescence.

Hot cathode discharges are common plasma sources for fundamental plasma physics studies and other applications due to their quiescent and relatively simple properties, and tungsten filaments are commonly used for the ease of heating them. Recently, tungsten filaments are increasingly being replaced by less luminous alternatives, such as barium oxide or lanthanum hexaboride. These materials can emit electrons at temperatures close to 1000 K lower than tungsten, greatly reducing their blackbody radiations. This results in significant improvement in signal recovery for active spectral diagnostic, such as laser-induced fluorescence. However, these less luminous cathodes often come in vastly more complicated designs than those of tungsten hot cathodes and are much more expensive to procure and difficult to operate. In this paper, we present a simple, low cost direct current heated design of a LaB6 cathode that is manufactured at suitable dimensions and make a comparison of the laser-induced fluorescence (LIF) signal-to-noise ratio of this LaB6 hot cathode discharge with that of a typical tungsten filament discharge, revealing that LaB6 has, indeed, an improved LIF signal-to-noise ratio compared with the tungsten filament.